ABSTRACT
Objective: To determine the components of 3'-hydroxy puerarin, puerarin, daidzin, daidzein, genistein glycosides, and genistein from Pueraria Radix and to study the quality of medicinal materials combining with anti-oxidant activity. Methods: The contents of six main components from Pueraria Radix samples were determined by using UPLC-DAD method, which performed on AgiLent Zorbax SB-C18 (5 mm×2.1 mm, 1.8 μm), acetonitrile and 0.1% acetic acid aqueous solution as mobile phase, column temperature 30 ℃, and the flow rate of 0.4 mL/min. The anti-oxidant activity evaluation of DPPH was accomplished by using UV spectrophotometry. Comprehensive weighted evaluation was based on seven indicators, combined six kinds of ingredients with the IC50 of medicine, and made cluster analysis. Results: Studies had shown that the quality of No. 8, 9, 50, and 51 samples of Pueraria thomsonii was better than others, which collected from Jianyang and Jiangyou. And the quality of No. 19, 20, 29, 30, and 31 samples of cultivated-planting Pueraria omeiensis was poor. The quality of No. 33 ~ 37, 65 ~ 69 samples of Pueraria Radix was better than other places in Sichuan Province, which collected from Pingwu, Beichuan, and Qingchuan. Conclusion: The UPLC wavelength switching technology multicomponent simultaneous determination method is simple and convenient, which has good reproducibility, strong specificity. Comprehensive weighted evaluation method can analyze the quality of medicinal materials better, which provides references for comprehensive evaluation on the main varieties of Pueraria.